Your English writing platform
Discover LudwigExact(1)
By contrast, strong staining was present in the subperiderm, a layer of embryonic periderm-related cells that is specific to archosaurs (Crocodilia and Aves) [ 11].
Similar(59)
After the second round of infection, cells were trypsinized and seeded onto a layer of mouse embryonic fibroblasts cell (MEF) feeders in a 10 cm culture dish using hES cell medium.
Placodal tissue is a homogeneous layer of embryonic ectoderm that becomes specified early in development to form a number of different tissues.
ES cells were grown on a layer of male mouse embryonic fibroblast (MEF) feeder cells.
NSCs were infected once with lentivirus expressing Oct4 and Nanog (ON) and plated onto a layer of irradiated mouse embryonic fibroblasts (MEFs) in human ESCs (hESC) medium [25].
The results are displayed as means (±S.E .. Murine embryonic stem cells (D3) were cultured on a layer of mitomycin treated mouse embryonic fibroblast feeder cells.
R1 (129Sv) embryonic stem (ES) cells were grown on a layer of G418 (Invitrogen) resistant embryonic feeder cells in a medium supplemented with leukemia inhibitory factor.
The embryos were then placed on a layer of mitomycin C-inactivated embryonic fibroblasts (feeder) in four well plates (individual one embryo per well or two to three embryos per well) in stem cell medium (see below).
iPSCs were maintained on a layer of mitomycin C-inactivated mouse embryonic fibroblast (MEF) with cDF12 medium or on Matrigel (BD Biosciences) with mTeSR1 medium (Stem Cell Technologies) as previously described (Liu et al., 2014; Ding et al., 2015; Fu et al., 2016).
The most widely used systems for maintaining hPSCs persist from the early days of hPSC derivation and rely on a layer of either mitotically-inactivated mouse embryonic fibroblast (MEF) feeder cells (see Figure 2 A and D) or complex extracellular matrix (ECM) extracts including MatrigelTM and GeltrexTM [ 1, 36].
The murine ES cell line R1 (Nagy et al, 1993) was routine cultured on a layer of mitomycin-C-arrested mouse embryonic fibroblasts in ES medium: DMEM-Glutamax supplemented with 15% FBS, 25 mM HEPES pH 7.4, 1.2 mM sodium pyruvate, 0.12% monothiolglycerol and 1000 U/ml recombinant leukaemia inhibitory factor (LIF; Chemicon International, Temecula, CA) and passaged every second day.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com