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Invasive breast cancer and other tumors of epithelial origin must breach a layer of basement membrane (BM) that surrounds the primary tumor before invading into the adjacent extracellular matrix.
Capillaries consisted of a single layer of endothelial cells surrounded by a layer of basement membrane, forming an intact BBB.
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The role of LOXL2 in 3D development of normal breast epithelial cells was assessed by using the well-characterized MCF10A acini assay [ 14], in which cells are cultured on top of a layer of recombinant basement membrane (Matrigel) within a Matrigel suspension.
Moreover, electrical reduction of PPy(DBS) with NSCs lead to a loss of cell viability, which was effectively prevented by coating the polymer films with a gel layer of basement membrane matrix prior to cell culture.
The ability of HUVEC to form capillary-like structures on a 3D layer of basement membrane (Matrigel) was tested (Belotti et al, 1996; Taraboletti et al, 2002).
The accumulations of collagen fibrils in cellular indentations, and the occasional presence of an incomplete layer of basement membrane-like matrix on the cell surface manifest the enhanced matrix production by the cells (Fig. 9).
Cells were plated onto a thin, pre-solidified layer of basement membrane matrix (Matrigel; BD Bioscience) and grown for 3 4 days in cell culture media containing 2% matrigel.
So he did what any pragmatic romantic would do: he power-sprayed out a layer of festering sludge in a basement chamber, filled it with water and took a swim.
Human breast luminal epithelium and the stromal compartment are separated by a layer of myoepithelial (ME) cells and basement membrane, whose disruption is required for tumor invasion.
The mature epidermis is composed primarily of keratinocytes arising from a layer of basal cells situated on the basement membrane.
Laminin is a cross-shaped ECM protein ubiquitous throughout the basement membrane, a layer of proteins that support epithelial cell growth and provide a molecular barrier between organ tissue and capillaries or organ compartments.
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