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A later determination by Clarke in 1880 reflected the several geodetic surveys that had been conducted during the interim.
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A validation study comparing prospectively determined time to pregnancy and a later retrospective determination, with a median recall of 14 years, found 80% sensitivity and 95% specificity for infertility (time to pregnancy of ≥ 12 months) (Joffe et al. 1993).
Recruitment will run for a 12 week period and will allow for later determination of the percentage of parents in the general community that are likely to take part in an online program if suggested by their MCH nurse (birth rate data for this region, throughout this time period will be compared to our sample).
Inter-batch coefficient of variation was 8.2% A whole blood aliquot was stored at −80°C for the later determination of MTHFR genotype.
When species determination was not possible by sight, bumble bees were caught with a net and either determined to species on site or brought to the laboratory for later determination.
At delivery, umbilical cord blood was collected for the later determination of serum ionized magnesium levels.
Dried shoot material was milled for later determination of total N and P concentration.
A digital photograph was taken from one side of the head from each parent kestrel for later determination of coloration of the cere.
At the end of each experiment, hearts were rapidly freeze-clamped with tongs cooled in liquid nitrogen and stored frozen at −80°C for later determination of enzyme activity.
Before sacrifice, venous blood was drawn for blood culture and serum samples (≥500 µl) were stored at −20°C for later determination of anti-PVL antibody and C-reactive protein titers.
Fish were bled by caudal puncture into heparinized tubes and the plasma, collected after centrifugation (6000×g for 10 min), was stored frozen at −70°C for later determination of plasma cortisol and glucose levels.
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