As published, Mt3.5 (with the last annotation release Mt3.5v5) contained 62,379 annotated gene loci with 14,309 additional predictions classified as transposable element-related.
Keeping these reserves in mind, as a first approach, we have screened candidates using the last annotation releases from both CRIBI and NCBI and selected a set of genes showing positive expression patterns in targeted organs, thus lowering down the candidate gene number to 10 to 65 per QTL.
Cancer-specific survival was recorded from sampling visit and until the last annotation of the patient being alive.
In case of bladder cancer death, survival was recorded from the date at which tissue was collected and until the last annotation of the patient being alive.
A last look at functional annotation is that of transporter activity, of which each of the TRs and Goa1p is essential.
As a last step, the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway approach for higher order functional annotation was implemented using the tool DAVID.
Automatic annotation pipelines provide useful evidence of potential functions for genes and proteins, but in a last essential step, the scientist must manually evaluate the available information.
The last ten annotations comprised seven sequences with a complete ORF, and three with a prematurely interrupted ORF.
The last time annotations were updated was during Nov 2011.
The phylotype root must have a unique annotation, say A, which lasted until extant phylotype members that are annotated by A. Formally, within a clade with root r, every taxon x sharing the same annotation A as r, and for which A is conserved along the path from r to x, belongs to the phylotype defined by r and A (assuming the MRCA condition is fulfilled).
A component-anchored annotation (CAA) follows the last ANC, including its AAAs, but precedes the multiplier, if any is present in the component notation.
