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A larger virus, called Mimivirus, which lives in freshwater amoebas, turned up in 2003 and a few other, similar, viruses have been found since then.
In contrast, rabbits in the GDC54 inoculated group developed more severe clinical symptoms following challenge with a larger virus inoculum (6.93×104 genome equivalents).
This might be explained by an increasing virus population size – random sampling of a larger virus population increases the likelihood of detection of nucleotide changes, and increasing population size increases the probability that residue changes will be selected for.
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Anti-c-Myc specifically detected Myc-tagged LARGE in cells treated with the Ad-LARGE virus.
To examine whether there are non-α-DG targets for LARGE-mediated glycosylation, we infected DG-deficient neural stem cell clones with the Ad-LARGE virus.
To determine whether LARGE overexpression increased IIH6C4 immunoreactivity on the cells, we carried out immunofluorescence staining of neural stem cells that were infected with the Ad-LARGE virus.
Two days after infecting with the Ad-LARGE virus, Laminin-1 (Invitrogen) was added to the culture medium and incubated for 1, 6, and 12 hrs.
(A large virus repository is in Siberia).
Claverie says the findings fit with the idea that a large virus, perhaps similar to the Mimivirus, may have been the first to jump into a cell: The timing seems to fit, and the similarity between Mimivirus and eukaryotic genes involved in making proteins bolsters the hypothesis, he says.
We all knew that, given exposure to what has been assumed to be a large virus dose from an infected bird, people can develop severe H5N1 disease, with a very high death rate (345 fatalities out of 584 cases since 2003) [ 3].
Grant and Grigorieff improved this method further by studying how quickly a large virus-like particle called ' rotavirus double-layered particle' is damaged under the electron beam.
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