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A larger alignment of 831 CarD sequences is included (Source code 1 ).
This region of sequence divergence is also observed in a larger alignment [ 10] of EF-Tu molecules from Gram-negative organisms (data not shown).
We expect that a more efficient pairwise potential optimization algorithm that can search a larger alignment space will further improve the alignment accuracy.
After every sequence has been either placed in an alignment or placed as a "singleton," the individual alignments are then profiled to a larger alignment.
This means that, should both genes be concatenated to form a larger alignment, different context-dependent codon partition models may need to be used for each gene to perform accurate phylogenetic reconstruction for such a concatenated alignment.
To improve resolution of GLDH phylogeny, an individual phylogenetic analysis was performed using a larger alignment (302 amino acids) with greater taxonomic sampling (151 sequences), although relationships between major taxonomic groups remain poorly resolved.
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Multigene concatenation proposes that phylogenetic analysis should always be performed using all available character data, essentially sticking many aligned genes together to give a large alignment.
Because ML phylogenetic inference is NP complete [6], no practical method can guarantee that it will find the optimal topology for a large alignment.
To reduce the cost resulting from a large alignment target, many algorithms have been developed that rapidly reduce the size of the search target for aligning a given read.
The authors then proceed to identify sets of mutations that might be suitable drug targets, through an analysis of covariation in a large alignment of HIV sequences.
We found a large alignment between CardioNet and both mitochondrial networks with 90.48% and 92.49% of the mitochondrial network reactions are represented in CardioNet.
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CEO of Professional Science Editing for Scientists @ prosciediting.com