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In Texas, in other words, you are prohibited from walking down the street with a small revolver in a holster on your hip but entitled to be out in public with a large shotgun slung over your shoulder.
A total of 2 μg of ligated metagenomic DNA was electro-transformed into the E. coli TG1 electrocompetent cells to obtain the pilot shotgun library, while the rest of the ligation mixture was used in 27 separate transformation reactions to generate a large shotgun library and overcome a problem of promiscuous (fast growing) clones.
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Our strategy was to employ a simpler version of the approach used by TIGR to assemble large shotgun sequencing projects [20].
Due to the enormous quantity of short DNA fragments in large shotgun sequencing-derived or pyrosequencing-derived metagenomic datasets, methods have been developed that are more suitable for taxonomic binning than the analysis of highly conserved phylogenetic marker genes.
In summary, our results suggest that the design of large shotgun proteomics studies should focus on efficiency not only to save resources but, most importantly, to yield reliable peptide discoveries.
"A lot of media traditionally have these large shotguns, and we've got these high-powered rifles".
I heard a gun being cocked, and suddenly all eight cops poured around the corner, racing out after this guy the last of whom was a giant, seven-foot-tall black guy carrying the largest shotgun I've ever seen.
Hence, we designed a large collection of simulated shotgun metagenomic datasets to assess the effects of read length, phylogenetic method, number of reads, and reference database size and diversity upon the construction of read trees.
Typically, the image of a mature whitetail doe, or a buck, appeared on a large flat-screen television, the shotgun scope's crosshairs tracking its movements.
As they opened it, they saw a large meat cleaver, a gun, shotgun cartridges and an improvised explosive device (IED) made out of pipe capped at both ends.
A large proportion of the total shotgun reads (14.6%) had BLASTN alignments >500 bp with the Thiomicrospira crunogena XCL-2 genome [12].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com