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Taking a large-scale screen on available TCS from various databases as our basis (see Supplementary material), we considered three general scenarios spanning from local to more global changes of TCS: (i) Individual amino acid changes.
Based on a large-scale screen for miRNA expression patterns associated with distant metastasis Foekens et al. [17] were able to show that miR-516-3p miR-516-3p miR-516-3ps the expression of which is associated with an adverse prognosis in estrogen receptor-positive, lymph node-negative primary belongscancer [18].
We performed a large-scale screening for LUC activation on T-DNA tagged cell cultures subjected to LT stress in different developmental stages of plant regeneration in vitro.
Before embarking on a large-scale screen, it is important to develop an assay that is sufficiently robust to allow the generation of meaningful results.
We believe that a large-scale screening should ideally be based on the detection of one single analyte.
The first large-scale screen on Candida albicans was conducted to identify complex haploinsufficiency in the genetic network in order to understand the morphogenic transition between yeast and the filamentous growth in this human fungal pathogen.
Large scale screening of pharmaceutical and nematicidal compounds on C. elegans can now be achieved when liquid CeMM is used with equipment for automated culturing [ 8] and experimentation ([ 9], updated version available from Union Biometrica).
Given the beneficial effects of exercise on mammalian immune function [54] [56], zebrafish can be used as an exercise model to investigate exercise-induced stimulation of immune function and fast large scale screening for the potential beneficial effects of exercise on various diseases.
A detection strategy for such rare metabolic disorders, which is currently being explored on a large scale, is by screening the enzyme defects in dried blood spots (DBSs) collected from entire newborn populations.
Therefore, it is a feasible approach for large scale screening.
A recent endeavor in functional identification and prediction of novel lncRNAs in rice on a large scale by screening of a mutant library has been a promising example [44].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com