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We constructed such recombinant yeast strain, which could be used for a large scale preparation of edible vaccine for animals based on the functional analysis of mammalian myostatin in our previous report [ 6].
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Compared with the existing methods, the method demonstrated here was facile but effective and could be readily used for a large-scale preparation of the PANI/Au.
Compared with the existing methods, the method demonstrated here is facile but effective and could be readily used for a large-scale preparation of this type of composites.
More significantly, the oxidative synthesis method might be superficial but effective and could be readily used for a large-scale preparation of the PPy silver nanocomposites, representing a potential application in constructing the nanocomposites of metallic nanoparticles with conducting polymers.
This was followed by a large-scale preparation of pure alkaloids using the above determined conditions.
Biochemical investigation of the age-associated changes often requires a large-scale preparation of sufficiently pure and terminally senescent cells.
In this study, we demonstrate a novel strategy for a large-scale preparation of active caspase-3 in E. coli.
At least 29 MPs, as judged by their higher productivity compared to GFP, might be suitable for a large-scale preparation.
Once the requirement had been met, a large-scale preparation of plasmid DNA was extracted; the DNA plasmids were named shRNA-WJ4 (the RNAi plasmid), shRNA-HK (the nagetive control plasmid), respectively.
A novel method for a large-scale preparation of active caspase-3 was developed by a strategic engineering to lack auto-activation during expression with amino acid sequences susceptible to thrombin, facilitating high-level expression in E. coli.
This research represents the first example to highly express a full-length caspase-3 as a soluble protein in E. coli, facilitating a large-scale preparation of active caspase-3.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com