Since our study examined the distribution of allelic frequencies on a larger scale (markers were spaced about every 10 Kb in genes) and the regions found are much larger (average size = 107 Kb, 95% C.I. = 5 977, from a lognormal distribution), recombination cold spots are unlikely to explain the regions we have discovered.
It is a large-scale marker that is not limited to a specific taxonomic level and has even been used in mega-systematics analysis [ 15].
The massive power of the NGS technology for rapid and large scale marker discovery laid the foundation for the super-fast development of markers linked to the target gene Lanr1 demonstrated in this study.
The markers developed by the strategy described in this study are all co-dominant SNP markers, which can readily be converted into high throughput multiplex format or low-cost, simple PCR-based markers desirable for large scale marker implementation in plant breeding programs.
These sequences provide a powerful tool for developing markers in a large scale.
This study was the first to develop SNP markers on a large scale to construct a genetic map for cultivated peanut.
Our work represents a large scale development of new genic-SSR markers for application in genetic improvement, complementing the approximately 2,000 SSRs identified in BAC-end and sequence scaffolds of DH-Pahang [ 15].
The validation of the erbB2 detection in a large scale population thus represents a new prognostic marker that can be used as a sign of EC early warning, and for the prediction of the disease progression.
Hence, the PLUG system is an effective tool for large-scale marker development.
Consequently, SLAF-seq is an ideal strategy for large-scale marker developing and genotyping for species which is lack of reference genome information, such as wax gourd.
In this study, a SLAF-seq was used for large-scale marker discovery and genotyping to develop a high-density genetic map.
