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A large scale expression and subsequent membrane preparation was made in order to analyse whether overexpression affected membrane localisation of heterologous protein.
Furthermore, the mitochondrial system is still being investigated for novel insights into disease mechanics, where a large scale expression analysis (as presented here) provide useful insights.
One positive colony was selected to a large scale expression in a 3 litres BioFlo 110 laboratory fermenter (New Brunswick Scientific).
To identify genes associated with Al stress and tolerance in wheat, a large scale expression profiling study was initiated using the Affymetrix GeneChip® Wheat Genome Array which allows the screening of 55052 transcripts.
Looking at the other relevant studies for cancer transcriptomics, a large scale expression study presented by Wang et al. identified a set of gene markers for prediction of metastasis for breast cancer [ 14] and followed by Chari et al. demonstrated an approach based on multiple concerted disruptions (MCD) analysis and identified genes and pathways in cancer [ 15].
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In order to determine which of the two combinations above gave the best overall yield, a larger scale expression was carried out with a representative antibody which showed optimal expression with each of the two combinations; 4G2 for 20 ng/ml inducer and ET149 for 50 ng/ml inducer.
We designed a large-scale expression experiment focussing on the influence of sequence variations in the translated region (TR) of the mRNA without changing the 5′-untranslated region (5′-UTR).
In addition, we have carried out a large-scale expression and purification of recombinant hGM-CSF in B. mori pupae and developed a new approach to the oral administration of these recombinant proteins [20].
The PCC cutoff value of 0.78 was then chosen to screen significant co-expression correlation from a large-scale expression data set.
Subtype classification as exemplified by the five-type classifier does not sample all of the signatures available in a large-scale expression profiling analysis.
The membrane fraction from a large-scale expression was resuspended in buffer A [150 mM KCl and 50 mM Tris (pH 8)] with protease inhibitor phenylmethanesulfonyl fluoride (PMSF, 1 mM) and homogenized.
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