A laborious and technically difficult cell culture cytotoxicity assay is still regarded as an alternative reference standard, but is seldom performed in most microbiological laboratories.
Obviously, this is a laborious method and difficult to apply to the whole country as it is an exceptionally hard task to evaluate all hospital records for AFP cases, especially since Iraq does not currently have reliable systems for retrospective patient data review [ 13, 19, 21].
Even though evaluation of community-based health interventions involving a comparison city as a control region can be laborious and difficult, such approaches are necessary to garner future support from policy-makers and other key stakeholders.
Although the procedure was laborious and difficult to reproduce manually, a 30% higher cell density was obtained and the product related mRNAs showed higher expression, such as the mRNAs encoding alcohol oxidase 1 (AOX), formaldehyde dehydrogenase, yeast PDI, type II procollagen chain and the C-P4H α(I) and PDI/β subunits.
Finally, in Phase 3, the role of Gordon Sweet's Silver staining was determined for the assessment of a third set of cases, to see if this laborious and difficult staining procedure is even necessary.
However, sequencing remains a laborious and expensive technique, making it difficult to obtain answers concerning the genetic composition of serotypes, or newly emerged variants of interest in a timely manner.
Furthermore, it is very laborious and difficult to find the cause of such nuisance tripping in RCCBs.
Thus, screening of direct substrates for specific kinases is still laborious and difficult.
The extraction of more homogeneous cell populations, however, which is often laborious and difficult to standardize, involves manipulation of the cells and may influence the expression profiles [ 6- 9].
The size of the mapped regions of these 17 diseases varies between 0.6 and 7.75 megabases (figure 1) and renders mutational screens for disease association laborious and difficult.
