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Determination of homocysteine was carried out by using a kit based on immunoassay (Abbott Laboratories, Ltd., Pakistan).
Ammonia was determined with a kit based on the reductive amination of 2-oxoglutarate using glutamate dehydrogenase and NADPH.
The quantitative determination of CRP was carried out using a kit based on nephelometry (Dade-Behring N High Sensitivity CRP).
A kit based on luciferin/luciferase assay has also been recently used by other researchers for determination of ATP levels [ 32, 33].
A kit based on the Bradford method (Nanjing Jiancheng Technology Co., Ltd., Nanjing, China) was used to measure tissue protein content.
A kit based on ICA should be a reliable and rapid method for clinical diagnosis and, even in the hands of inexperienced personnel, easy to perform under harsh field conditions.
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In preparation for the trip, Catalano packed a medical kit based on a checklist that Galea had made for such trips.
AGP was measured using a commercial kit based on a radial immuno-diffusion assay according to the manufacturer's instructions (The Metabolic Institute, Tokyo, Japan).
ELISA was used to detect secreted VEGF-A in vascular SMC culture media using a VEGF ELISA kit based on the sandwich enzyme immunoassay technique (R&D Systems, Minneapolis, MN, USA).
When an ELISA kit, based on a polyclonal antibody against irisin, is used to assay a complex protein sample such as plasma or serum, the signal will include that from any irisin but also that from cross-reacting proteins.
A future kit based on Flora will create video displays on the back of a jacket, says Phillip Torrone, a senior editor at Make magazine and creative director at Adafruit.
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