By ensuring a key spindle-length regulator is present in rate-limiting amounts or absent, the cell can modify the shape of the spindle.
This shows mitotic morphologies, as well as a set of quantified key spindle and cell parameters, that are indistinguishable from controls.
10.7554/eLife.02875.013 Figure 7. Key spindle and cell features in APs are not altered by 30 pM nocodazole.
Second, key spindle parameters, such as its area and the amount and distribution of microtubules, were not significantly affected.
Kinesin spindle protein (KSP) is a mitotic kinesin that is expressed only in proliferating cells and plays a key role in spindle pole separation, formation of a bipolar mitotic spindle, as well as centrosome separation and maturation.
Other gene associated with RMS development is BUBR1 (budding uninhibited by benzimidazoles 1 homolog beta -a key protein in the mitotic spindle checkpoint and potent inhibeta -af Cdc20.
DOI: http://dx.doi.org/10.7554/eLife.02875.008 Previous studies have shown LGN to be a key player in spindle orientation.
However, the molecular identity of the signal(s) provided by sensory axons had not been defined, and our data indicate that Nrg1 provides a key signal for spindle maintenance.
For example, NUSAP1, the gene with the highest number of connections to other nodes (or degree) in our network, is a key gene for spindle microtubule organization and has been identified as playing a central role in regulating mitosis depending on its phosphorylation state [ 30, 31].
In addition to mediating the biological function of selective cargo transportation on actin cables, myosin-X directly interacts with microtubules and has a key role in spindle assembly during meiosis to ensure faithful delivery of replicated chromosomes to daughter cells following cell division (Weber et al, 2004; Woolner et al, 2008).
