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In brief, we determined the quantity of the template at the Ct (target cycle) and the efficiency (X) by a internal standard control and the fold increase was calculated as X inputCt-IPCt).
The supernatant were recovered, evaporated using a Speed Vac (Thermo Fisher Scientific) and resuspended in water 0.4% acetic acid and 2 ng mL−1 of docosahexaenoic acid in methanol as a internal standard (1 1, v/v).
Similar(58)
A-86093 was used as an internal standard (I.S).
Rhodium was then added as an internal standard to a final concentration of 10 μg·L−10
This ladder proved to be important as an internal standard for a correct allele assignment.
Transcript level differences were calculated by a relative quantification approach using an internal standard gene [34].
As an internal standard 1 µl of a 1 mg/l Rb standard solution was added.
A standardized quantity of gallium was added to all samples as an internal standard.
Dodecane was used as an internal standard.
They used propranolol as an internal standard.
Ribitol was added as an internal standard.
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