Interestingly, S. cerevisiae Far8 shares sequence similarity with the striatins, and Tpd3, a PP2A-A orthologue, has been found as an interacting partner with Far11 in yeast two-hybrid assay [ 19].
We have found that rhodopsin serves as an interacting partner and a vehicle for ciliary delivery of a key phototransduction protein, GC-1.
A neurally differentiated embryonal carcinoma-derived protein (Necdin) was isolated as an interacting partner for Gαo from a mouse brain cDNA library using yeast two-hybrid screening.
Plants were therefore generated expressing a steroid-inducible version of BLR in the form of a BLR-GR fusion regulated by the 35S CaMV promoter which would result in nuclear localisation of BLR in a steroid inducible manner, showing independence of an interacting partner.
In PCA, a reporter protein, such as a green fluorescent protein (GFP) variant [ 4, 5] and a luciferase [ 6- 10] is dissected to a split from, and each split reporter protein is fused to an interacting partner.
However, silver staining of the gel for the immunoprecipitates after transfer revealed a band with a molecular weight of approximately 300 kDa in the adenocarcinoma, suggesting APC as an interacting partner with β-catenin in this tumour, in addition to Lef-1 (data not shown).
The LITE system includes two parts: a customizable TALE DNA-binding domain, fused with light-sensitive cryptochrome 2 (CRY2), and transcriptional regulator-fused CIB1 (an interacting partner of CRY2).
One was the "grin face" or "social grimace": the corners of the mouth and the lips are retracted to expose the gums, the jaws are closed, there is no vocalization, body movement is inhibited, and the eyes are directed toward an interacting partner.
It is possible that an interacting partner(s) in the nucleus masks the p68 NESs sequences.
ASPP2 was previously identified as an interacting partner for several proteins, for example, Bcl-2 and p53.
