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Expression of mCherry reporters containing poly(A) inserts of various lengths in the presence and absence of RFs.
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(D) Clones were randomly selected to detect the percentage of clones with a phagemid containing an insert of a proper size for a VHH.
Subsequently, the subgenomic DNAs were cloned into pGEM-T (Promega, Madison, WI, USA) to form a plasmid designated pT1P1-5′3′-UTR-I pT1P1-5′3′-UTR-I pT1P1-5′3′-UTR-I pT1P1-5′3′-UTR-I
Additional necessary control for the Luciferase experiments is a pGL- SV40) pGL- SV40vector harboring an insert of a scrambled sequence that its size range mimics the average insert size of the tested ECRs.
The nadir of visual intelligence is plumbed when we hear a telephone ring and the director Bryan Foy cuts to an insert of a vibrating phone bell, proudly confirming that he had indeed recorded a real telephone.
This is an insert of a patient vomiting.
Kasdan then cuts to an insert of a piece of carpet, and the panties are dropped in from above frame.
A plasmid containing an insert of M. bovis-hspX-M.
Sensitivity was estimated at about 10 molecules per tube of a plasmid containing an insert of the first amplification product.
In addition, a cDNA clone containing an insert of approximately 1.4 kb was obtained by screening an H69AR cDNA library with 125I-MAb 3.186.
Screening of a heart cDNA library in order to find the whole-length transcript resulted in identification of a positive clone with an insert of size 1.3 kB.
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