A protein complex at an initial time has no explicit connection with itself at subsequent times.
So, when you are trying to solve elapsed time, you will always need to have an initial time and a final time.
Following an initial timed wash with buffer A, peptides were eluted using a linear gradient from 5 80% buffer B over a 30 min interval.
The oven temperature was programmed from 140 to 245°C at a rate of 4°C/min after an initial time hold of 5 min and the final time hold of 15 min (the total analysis time was 45.00 min).
The DNA unwinding kinetics show an initial time lag followed by an increase in fluorescence in two phases, a fast phase and a slow phase.
After an initial time period of 0.5 min at 95%% eluent A, the percentage of eluent B was linearly raised to 15%% until 6 min.
Cross-tabulation matrices show comparisons among three maps: 1) the reference calibration map of an initial time, 2) the reference validation map of a subsequent time, and 3) the model's predicted map of the same subsequent time.
Ions are introduced by gated pulses, conventionally via a Bradbury Nielsen gate, into the drift tube to establish an initial time to separate ions by their drift times.
It had an initial time to maximum concentration of 1 6 hours, a secondary maximum of 6 8 hours, and a tertiary maximum of 24 hours.
Two days post infection, a fraction of the infected cells was harvested for an initial time point (T = 0), and puromycin selection (using cell line-specific levels previously determined by killing curves) was then initiated for the remaining cells.
For every collection point, a set of n g a t e s measurements are derived, corresponding to integration from an initial time t G up to t G + Δ t, where Δ t is the width of the time-gated window.
