Sentence examples for a human trail from inspiring English sources
A human TRAIL cDNA fragment (corresponding to amino acids 114 to 281) obtained by PCR was cloned into the pET-23d (Novagen, Madison, WI, USA) plasmid, and expressed protein was purified using the His-band Resin and Buffer Kit (Novagen) [ 13].
To determine if endogenous TRAIL plays a role in the apoptotic process that occurs as a consequence of culture in DM, we utilized a soluble human TRAIL decoy receptor chimera (TRAIL R4:Fc) which functions by binding endogenous TRAIL and preventing its association with the endogenous TRAIL receptor.
A replication-deficient adenovirus encoding the human TRAIL gene (Ad-TRAIL) expressed from the CMV promoter was generated as described [ 14] using human TRAIL cDNA purchased from the Riken BioResource Center (Tsukuba, Ibaraki, Japan) [ 15].
A replication-deficient adenovirus encoding the human TRAIL gene (Ad-TRAIL) expressed from the cytomegalovirus promoter was generated using standard methods as previously described.
In this report, we examine the activity of HGS-ETR1, a fully human TRAIL-R1 agonistic mAb, and our results confirm and extend these previous observations.
The organic formation of the human trail system between an origin and a destination node (i.e., point) has a bottom-up nature.
To further highlight these results, we analysed cell surface expression by immunofluorescence staining using an alternative specific antibody against human TRAIL.
Specificity of TRAIL staining was confirmed by antibody absorption, using recombinant human TRAIL, according to a method published previously [ 34].
In our in vitro studies, activation of TRAIL-R2 with recombinant human TRAIL resulted in a robust inhibition of insulin-stimulated glucose uptake and insulin-stimulated de novo lipogenesis.
Results: Preincubation with NVP-AEW541 surprisingly decreased apoptosis induced by recombinant human TRAIL (rhTRAIL) or an agonistic DR4 antibody while sensitivity to an agonistic DR5 antibody was increased.
Previously, we cloned the human TRAIL promoter and identified a number of putative transcription factor binding sites including NFAT and Sp1 sequences [21].
