IORT implies delivery of a high, single dose of radiation to a limited intrasurgical anatomic area.
One of the interesting candidates from the previous study, SNCG, was markedly increased by a high single dose of 10 Gy but not by fractionated irradiation.
The mice were orally treated for 5 days; then a high single dose of MnCl2 (100 mg/kg) was given by intraperitoneal injection to the mice.
In fact, experimental diabetes during pregnancy, induced by a high single dose of streptozotocin, occurs by direct toxic effects on pancreatic β-islet cells [ 23].
Those of the Mn group were given an intraperitoneal injection with a high single dose of manganese chloride (MnCl2, 100 mg/kg, the pH value was close to 7.0) without oral B401 treatment.
Mice of the B401+Mn group were orally fed with the B401 formulation (50 mg/kg, the pH value was close to 7.0) twice every day for 5 days and then treated with a high single dose of MnCl2 (100 mg/kg, the pH value was close to 7.0) by intraperitoneal injection.
Since no MTD was achieved, it is possible that higher single doses of RGD-A-TNF may also be safely administered.
An exception is the recent study conducted in mice using a relatively high single dose of bleomycin which was able to detect measurable differences in lung function for up to 6 months after bleomycin administration [ 12].
The recent literature contains a number of reports of the use of a higher and single dose of rFVIIa to achieve bleeding control in haemophilia patients with inhibitors.
To assess the therapeutic effect offered by a higher dose of WNIG, a single dose of 8 mg/mouse was given on days 3, 4, or 5 after infection.
