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Although this observation confirmed that a decreased CodY response, that is a higher repression of transcription of CodY-regulated genes, improves protein overexpression, this strategy would compromise the chemically-defined nature of GCDM.
An over-expression of an Argonaute gene, as observed in bm3 and AS225 deficient COMT plants, is considered to induce a higher repression of target genes.
The repression index is defined as the ratio of 'no PRE' to 'PRE' pigment levels at each site, thus a higher repression index indicates stronger silencing.
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Our results are supported by a human study that reported that highly conserved miRNAs had higher repression efficiency (repression level and amount of targets) as compared to less conserved miRNAs [ 45].
Alternatively, rapid early platelet decline may represent higher repression of megakaryopoiesis, or increased platelet consumption.
siFLuc-II also showed significantly higher repression at the coding region of the FLuc segment.
Conversely, the two siRLuc showed higher repression when the RLuc segment was in the 3′-UTR.
This study elucidates whether the coding region or 3′-UTR elicits higher repression.
Results showed that the two siFLuc elicited higher repression when the FLuc segment resided in the coding region.
The siFLuc-I against the coding region showed significantly higher repression than that against the 3′-UTR.
The binding sites located in the 3′-UTR, where the interruption by working ribosomes is avoided, rendered higher repression.
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