The phrase "a higher level of stringency" is correct and usable in written English.
It can be used when discussing increased strictness or rigor in rules, regulations, or standards.
Example: "The new policy introduces a higher level of stringency in safety protocols to ensure employee well-being."
Alternatives: "an increased degree of strictness" or "a greater level of rigor."
While the quality and quantity of EST data varies greatly for each species, we have found that using a high level of stringency (p = 90 or 95) tends to prevent over assembly and helps distinguish between gene family members.
However at the higher level of stringencies the size of conserved genome for large evolutionary depth was small and the number of SNPs reduces to a very small number.
This was a very strong response field and was amenable to a very high level of stringency so we focused on the Bonferonni corrected results with a FWER p-value cutoff of 0.00001 to insure that genes chosen for further analysis were reliably responsive to our experimental conditions and to keep the number of genes in the downstream analytical pool reasonable.
Thus, using a relatively high level of stringency, 12.5% of the GS20 454 sequences potentially identify novel maize transcripts.
Because of the high level of reproducibility that was achieved, a relatively high level of stringency (p < 0.0001) was chosen for selection of significant differences.
We used a relatively high level of stringency in this and the following case studies in order to focus on the more prominent changes in expression and to minimize false positive findings in the downstream analyses.
Overall, the high level of stringency and the potential to search a large library of broadly dispersed random point mutations are the two most likely reasons why we were able to identify subtle surface residues with important structural and functional properties.
MP and P10 were found to interact upto the highest level of stringency i.e., –Leu –Trp –His –Ade +X Gal as evident from the appearance of blue colonies on the plate similar to MP –VPg interaction, signifying a strong interaction between the two proteins.
We speculate that this high level of stringency (requiring the activation of four separate reporter genes) could explain why only a single clone was obtained.
As expected, no reads mapped at the highest level of stringency tested, as the requirement for 100% identity would preclude any matches.
