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Our results showed that only VEGF121 was weakly expressed in distant normal tissue whereas a high level expression of VEGF121 and VEGF165 in tumour tissues was observed (Fig. 1a).
Using the auto-induction method, we attained a high level expression of BoNT/C1(1 430) yielding more than 30 mg protein per 500 ml culture.
A high level expression of cyclin D1 (∼4 fold) and cyclin E (∼2 fold) was detected in MBP-1 knockdown HFF as compared to the control fibroblasts.
Interestingly, we found a high level expression of IL-32 in endothelial cells, including large and small vascular endothelial cells, with minimum or undetectable levels in most non-endothelial cells (Figure 1).
The P. pastoris expression system allows a high level expression of xylanases.
Transient transfection of 293T cells with this plasmid resulted in a high level expression of Z-eGFP protein (37.9 kDa).
In general, high FOXM1 levels were associated with a high level expression of all four of its target genes (in 12 out of 14 cases).
In contrast to the results observed in control cells, a high level expression of CBP2 occurred in infected samples in agreement with previous studies showing that HIV and HCV promote expression of collagen [ 33, 34].
Thus, the tumour suppressor function of caspase 2 may become a new option in therapeutic strategy aimed at control of tumour growth under a high level expression of HMGA2.
When the recombinant yeast was incubated at 28°C in a 1 L shake flask culture, a high level expression of endo-1,4-β-mannanase was obtained in Pichia pastoris (262 mg L−1).
Accordingly, in our study we observed that the E-cadherin/β-catenin complex was localised in the cellular cytoplasmatic membranes with a linear pattern and a high level expression of both molecules in the normal tissues used as controls.
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