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Dyer [70] reported that mellifera colonies show higher rates of colony activity or "worker tempo" than florea or dorsata and have a higher colony metabolic rate.
Since colonies containing an empty vector are larger than colonies expressing E4orf4, a higher colony size ratio represents a higher ability of the Ynd1 construct to mediate E4orf4 toxicity.
We observed that higher initial bacterial concentration resulted in a higher colony density in 1% agarose (Fig. 2g), while the colony density increased with culture time and reached to a maximum at t = 3 hours in 2% agarose (Fig. 2h).
However, a smaller initial density is correlated with a smaller difference in the number of surviving lineages (Fig. 5 a, Additional file 1: Figures S3 S4) and a higher colony roughness (Fig. 5 c).
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The longer trails may represent an adaptation to increase the robustness of the entire nest network: this is indicative of a higher, colony-level organization of polydomy (Cook et al. 2014).
Look for a high colony count (i.e., above 20 billion cfu) and a varied number of strains!
The SLiCE extracts from common RecA− E. coli laboratory strains such as DH10B, JM109, DH5α, XL10-Gold and Mach1 T1 supported the cloning of PCR fragments containing a 19-bp overlap region into the vectors, and were associated with a high colony formation rate (Table 1).
One clone had a high colony-forming ability in agar but failed to produce tumours in nude mice.
These cells express the proposed progenitor marker Notch-1 and possess a high colony-forming efficiency that was abolished when Notch signalling was blocked [ 2].
A high colony-formation capacity was also observed for the basal stem/progenitor cells of virgin mice (63 colonies per well), which is consistent with the notion that CD49fHigh cells contain a high proportion of basal progenitor cells as well as putative mammary stem cells [ 45].
Meanwhile, cells cotransfected with miR-203 and pcDNA-ΔNp63 plasmid exhibited a significantly higher colony forming efficiency, as well as a significantly shorter population doubling time than the control cells.
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