Exact(4)
A high viable cell density, which is indicative of the proliferative stage of the cords, was confirmed with H & E staining.
Besides, for a repeated transfection approach it is very important to maintain a high viable cell concentration during the whole culturing period.
The high productivity of the cell line in bioreactors can be said to be dependent on having a high viable cell density of rapidly dividing cells over an extended period of time.
Consequently, the fact that perfusion provides fresh nutrients while clearing part of the toxic by-products in spent medium contribute in maintaining a high viable HEK293SF cell population in the culture.
Similar(55)
There was an association (R2 = 0.36; p = 0.002) between a higher viable larvae ratio and lower Coxiella sp. burdens but not between the viable larvae ratio and Rickettsia sp. burdens (R2 = 0.08; p = 0.75).
On the contrary, GQ consumption yielded steady, but lower, qmAb values, in combination with an extended period of high viable cell densities.
A good cell line proliferative capacity and a high integral viable cell number (IVC) can result in high volumetric recombinant protein production rates.
The biopharmaceutical industry requires cell lines to have an optimal proliferation rate and a high integral viable cell number resulting in a maximum volumetric recombinant protein product titre.
In a first attempt to unravel whether the release of CK19 has a biologic function in tumor progression, we applied the CK19-EPISPOT to detect with a high sensitivity viable cells releasing CK19 in the BM of breast cancer patients.
At 24 h.p.i., significant levels of il1b were transcribed after a high-dose viable spore challenge (P<0.001) and of tnfa after low- and high-dose injection of viable spores (P<0.05 and P<0.001, respectively; Fig. 4A,B).
The use of CHO-K1 cells with a c-myc plasmid allowing for over-expressing c-Myc (designated cMycCHO) gives a higher integral viable cell number.
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