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Fearing for Mulder's life, Deep Throat helped Scully gain access to a high containment facility, where she managed to secretly remove a cryogenically-preserved alien fetus for use as collateral in saving Mulder.
The samples were then transported to a high containment laboratory; viral RNA was isolated and then introduced back into cells, by electroporation, to rescue infectious FMDV.
Nonetheless, reassortant viruses can be useful tools to evaluate immune responses raised to H5N1 HA and NA components in the absence of a high containment facility and special permits required to work with wild-type H5N1 viruses.
We performed an extensive survey of the occurrence of reagent contamination using conditions tailored to prevent other sources of contamination: a high containment facility, physical separation between pre- and post-PCR working areas and enzymatic carry-over prevention with UQPCR [12].
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He said part of the money would be used to build a high-containment laboratory where scientists could conduct research on dangerous pathogens.
But in a stroke of good luck, the university was about to construct a high-containment laboratory in Masopust's own building.
There, a lack of "standard operating procedures" led to dozens of employees being exposed to anthrax after a high-containment laboratory inadvertently sent live anthrax to labs with lower contaminant levels, where workers were not equipped to handle such dangerous pathogens.
The most likely source of their infection, the report concludes, is a batch of supposedly inactivated SARS virus that was brought from a high-containment facility into a low-safety diarrhea research lab where the two were working.
Next year, UTMB will start building a high-containment lab that can handle even the most dangerous viruses--one of only a handful in the U.S. With that capacity, and the expertise concentrated at UTMB, the new center should "make some very important contributions" to vaccine development, La Montagne predicts.
All of the described experiments were done in a Biosafety Level 3 laboratory, a high-containment environment recommended by the U.S. Centers for Disease Control and Prevention and the National Institutes of Health on an interim basis, whose use should become a permanent requirement for such experiments.
This procedure was performed in a high-containment Biosafety Level 4 laboratory at the Karolinska Institute (Stockholm, Sweden).
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