However, some sequence traces began as a single uniform sequence in the centromere-proximal end of the amplicon and transitioned to a heterogeneous sequence composed of double peaks as the sequence extended toward the telomere, suggesting that each homolog on that particular chromosome arm encoded distinct TLO sequences.
It is possible the plug adopts a variety of conformations in this sample (given the heterogeneous sequences of translocating nascent chains) or becomes disordered to allow translocation.
However, since most available sequences for this region are from cultured isolates, which are much less heterogeneous, sequencing of more clinical specimens is needed.
This is attributable to the heterogeneous sequence organization of PREs and low conservation of consensus sequences for the binding sites of PRE-binding proteins.
For example, PCR-based methods require highly multiplexed oligonucleotide pairs targeted to heterogeneous sequences with a range of melting temperatures and CG content to generate hundreds or thousands of amplicons in a single tube.
Arcady Mushegian, Bioinformatics Center, Stowers Institute for Medical Research The manuscript of Wong et al. presents good evidence that the transmembrane segments in proteins, which were thought to be compositionally biased and, because of this bias, not very useful in probabilistic sequence similarity searches, are in fact a heterogeneous group of sequences.
Transcription-induced chimerism (TIC) gives rise to chimaeric transcripts consisting of heterogeneous sequence segments originating from distant regions on the same chromosome or from two or more distinct chromosomes.
Secondly, without losing comparability, it allows the use of datasets with heterogeneous sequence lengths, which for instance arise from a combination of raw reads and assembled contigs.
Furthermore, in a mixture of heterogeneous sequences, certain bands may overlap, resulting in underestimation of viral complexity.
An approach to solve similarity problems could be the transformation of these heterogeneous sequences into uncertain temporal relations, obtaining a temporal constraint network.
It was not possible to establish an unambiguous sequence directly from the PCR product for every collection investigated as a number of the PCR products contained a heterogeneous mixture of sequences.
