Sentence examples for a heated stage from inspiring English sources
In order to visualize the dynamics of cofilin rod formation during cell stress, we used STHdhQ7/Q7 and STHdhQ111/Q111 cell lines stably expressing mCerulean cofilin fusion protein, and observed live cells over time during heat shock stress by fluorescence microscopy using a heated stage (Supplementary Material, Videos S1 and S2).
The new theater space, still called the Bessie in honor of the modern-dance master teacher Bessie Schönberg, has a larger stage, better wing space, twice as many seats and even a heated stage floor.
Micrographs were obtained using a Zeiss microscope fitted with a heated stage and a Hamamatsu Orca CCD camera.
Samples were observed under a Leica confocal microscope (SP2 AOBS MP) using a heated stage (Tempcontrol 37-2, Pecon) and 63× objective (zoom 4×) at 37°C.
Cells were then immediately placed on a microscope stage, equipped with a heated stage and CO2 circulator to maintain cells at 37°C and 5% CO2.
The plate was incubated for 15 min at 55°C and read on a heated stage at the same temperature as the Luminex 100 platform.
RAW cells (1×106 cells) were seeded on 25 mm diameter cover glass (Fisher Scientific), cultured overnight, then mounted in an Attofluor chamber (Molecular Probes) followed by imaging on a 37°C heated stage.
Dendritic cells were seeded at 3 × 10 per dish in pre-warmed media supplemented with 20 m M HEPES and transferred to a Zeiss Axiovert 200 inverted microscope with an enclosed, heated stage (Solent Scientific Ltd, Portsmouth, UK) with a maintained temperature of 37°C and left for 30 45 min prior to filming.
Coverslips were transferred to a 37°C heated stage in Leibovitz's L-15 medium (Invitrogen, Grand Island, NY) and filmed using a Nikon Eclipse 80i microscope using a 100× numerical aperture 1.40 plan apochromat oil immersion objective and an EMCCD (Andor technology).
Mice were anesthetized with 1.5% isoflurane (Abbot Japan, Tokyo, Japan) inhalation and placed in the prone position on an electric heated stage maintained at 37°C.
Prior to imaging, animals were positioned supine on an adjustable heated stage and sterile eye lubricant was applied to each eye.
