Sentence examples for a half volume from inspiring English sources

Each enzyme sample (3 μL/12 μL) was adjusted to desired pH by adding a half volume of buffer with different pHs: 0.2 M citrate buffer (pH 4.0), 0.2 M phosphate buffer (pH 5.0, 6.0, 7.0, 8.0, or 9.0) or 0.2 M CAPS buffer (pH 10.0).

Each enzyme sample (1.0 μg) was adjusted to desired pH by adding a half volume of buffer with different pHs: 0.2 M citrate buffer (pH 4.0), 0.2 M phosphate buffer (pH 6.0, 7.0 or 8.0) or 0.2 M CAPS buffer (pH 10.0).

PCR was performed in a 10 15 μl mixture containing 1 μM of each primer, a half volume of 2× Ampdirect plus buffer (Shimadzu, Japan), 1 μl of template DNA (or a small disc punched out from the FTA card), and 0.5 U of NovaTaq (Shimadzu, Japan).

Each enzyme sample (1.0 μg) was adjusted to desired pH by adding a half volume of buffer with different pHs: 0.2 M citrate buffer (pH 2.5, or 4.0), 0.2 M phosphate buffer (pH 6.0, 7.0 or 8.0) or 0.2 M CAPS buffer (pH 10.0, or 11.0).

A half volume of the aqueous layer concentrated to 50 mL (over 25 g) was subjected to gel filtration using a 1.5 L Sephadex G-25 (200 g, GE Healthcare Little Chalfont, UK) column (400 mL per fraction, more than 8 fractions).

A half volume of the cellulose cartridge eluent was dried and dissolved in 50 µL of 20 mM phosphate buffer (pH 7.0).

DNA was precipitated from the aqueous phase in the presence of 0.3 M NaOAc pH 7.0 and two and a half volumes of ethanol.

DNA was precipitated from the aqueous phase in the presence of 0.3 M NaOAc pH 7.0 and two and a half volumes of ethanol and followed by one 70% ethanol-washing and dissolved at 65°C for 30 minutes with 0.2 – 0.4 ml TE (10 mM Tris-HCl pH 7.4 and 1 mM EDTA and stored at 4°C till use.

Supernatants were transferred to centrifuge tubes (50 ml) containing a half-volume of polyethylene glycol (30%)/sodium chloride (1.6 M), and incubated at room temperature for 2 hr.

A half-volume of the PRP obtained was frozen at −30°C for 2 h and then thawed in a dry thermostat at 37°C for 30 min just before activation (frozen PRP).

Briefly, from day 0 to 7, sorted CD34+ cells were continually cultured in serum-free conditioned erythrocyte culture medium with 100 ng/mL SCF (Peprotech, Rehovot, Israel), 10 ng/mL IL-3 (Peprotech), and 6 IU/mL recombinant EPO (Recormon Epoetin beta, Roche) with a half-volume medium change twice a week.