It is also possible that the unlike lipid-lipid interaction Gibbs energies are actually somewhat smaller in a GUV than in a LUV.
Using optical tweezers, we pulled a thin tether from a GUV held in an aspiration pipette, that allows to monitor membrane tension through the measurement of the force F exerted on the optical tweezers' bead (Cuvelier et al., 2005).
Nevertheless, if the scaling of domain size with lattice size were exact, ∼3/4 of the lipid in a GUV with a composition BSM/Chol/POPC ∼1 1 1 would form a domain that would be visible by fluorescence microscopy.
Of course, Mirren went on to play the Queen, but this is the role – the female "guv" in the man's world of the Met – that made her a screen star after two decades in theatre.
"That song came out two weeks after we did our track," argues Wa'fande, who doesn't know the precise translation of "honest, guv" in Danish.
In an interview with India Today in February 1994 – perhaps significantly, a month after Kelvin MacKenzie's lengthy reign as Sun editor ended - he said it was, yes, "getting a bit old-fashioned - one day it will come out" (and sheepishly explained that it was nothing to do with him, guv – in 1970, "the editor [introduced] it while I was away").
The numbers of GUVs in a micrograph were averaged over all micrographs within the test and control samples.
In conclusion, we have demonstrated the trapping and stretching of GUVs in an integrated dual-beam optical trap.
There again, Letwin was the man who let a burglar into his house ("I just want a pee, guv") at three in the morning.
GUVs in mosaics were segmented using a computer-aided approach [ 58].
