This low level of GUS activity was confirmed using a GUS quantitative assay.
To analyze the promoter activity of UGT73C6 in different tissues and developmental stages a GUS reporter lines was constructed in which the GUS gene was expressed under control of the UGT73C6 promoter (UGT73C6 pro GUS).
To construct the ß-glucuronidase (GUS) reporter under the control of CRP4 promoter, a GUS coding sequence (plasmid pRT102) [ 55] was inserted downstream of the CRP4 promoter and introduced into pPZP221 binary vector [ 53].
To examine this interesting speculation, we conducted compartment plate assays using an A. thaliana Col-0 transgenic line stably expressing a GUS reporter gene fusion to the auxin responsive promoter DR5 (DR5::GUS).
A GUS DNA fragment was amplified via PCR with the primer pair, GUS (Additional file 1: Table S1), and was used as probe.
At Lever House in Midtown, the bar serves a Gus Meyer-lemonade with lemon vodka and mint.
"I think of her as a conceptual artist who is using story," she said, "but story in a way that you'd find in a Gus Van Zandt film".
But perhaps it shouldn't be a surprise, given that the director is Gus Van Sant, even if "Milk" often does not feel like a Gus Van Sant film.
A pair of male streetwalkers turn up in "On the Bus Mall," this time as bitter "kings among runaways" whose caustic world-weariness would not be out of place in a Gus Van Sant movie.
Indeed, Columbia's slate for December was crowded, with "Vertical Limit," a mountain-climbing thriller, and "Finding Forrester," a Gus Van Sant drama starring Sean Connery that many at the studio felt was Columbia's best Oscar prospect.
Sports is drama, and when you're lucky enough to witness a memorable moment, it's nice to have a voice to match — to have the accompaniment of a Gus Johnson during March Madness or an Andres Cantor during the World Cup.
