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(A ) Gross view of E11.5 Nf1 +/+, Nf1 GRD/+ and Nf1 GRD/GRD littermates.
(A ) Gross view of E11.5 Nf1 +/+, Nf1 +/- and Nf1 -/- embryos (B ) Isolated hearts from embryos in (A ) Black arrowheads point to two examples of blood-filled protrusions.
In addition, we used tile scanning to acquire a gross view of the vascular connections between the islet graft and the kidney parenchyma to demonstrate the graft host integration (Fig. 3D and E).
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P4 rACs displayed a drastic morphological change from a rounded, cobble-like shape of P1 cells) into the fibroblast-like appearance), confirming the dedifferentiation of rACs after 4 passages [ 30]. Figure 1(c) showed the gross view of beads encapsulated with rACs having a diameter of 2-3 mm and an integral spherical morphology.
This figure shows the gross view of the images presented in Fig. 5 E and F. Viewers can zoom in to specify the peri-graft Schwann cell network.
Regarding the kidney pericytes and Schwann cell network, Fig. 2C and D shows the gross views of the kidney microstructures and their association with the two cell types.
The gross view also shows a higher density of the GFAP+ Schwann cell fibers at the graft domain in comparison with that in the kidney parenchymal domain.
Fig. 6 shows the gross view and zoom-in examination of the overlap of the nestin-GFP and GFAP signals, confirming a subpopulation of the nestin-GFP+ cells as the GFAP+ Schwann cells.
Fig. 1C and D shows the gross view and zoom-in examination of the overlap of the nestin-GFP signals and the GFAP+ Schwann cell network of islet in situ.
Six weeks after islet transplantation, Fig. 5A D shows the gross view and zoom-in examination of the islet graft Schwann cell sheath.
Fig. 3B and C shows the gross view and zoom-in examination of the graft with fluorescence signals derived from the β-cells (insulin staining), blood vessels, and pericytes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com