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One of the most challenging problems in VAP is its correct identification, resulting from the lack of a "gold standard" method of diagnosis [1].
One of the strengths of this study is that diaphragm strength was tested by bilateral phrenic nerve stimulation, a gold standard method that provides a specific measurement of the capacity of the diaphragm to generate an inspiratory pressure [21].
Conclusion In our study, we cannot consider that one method of body composition analysis is more accurate than the other because we did not apply a gold standard method.
The limitations of this study are the lack of a gold standard method for comparison of ultrasound findings, the fact that we did not carry out clinical correlation, the small number of patients in the study and the lack of Doppler study of the nodules.
In other words, total quenching offers us a gold standard method to compare against for P. pastoris.
Furthermore, test evaluation is hampered by the absence of a gold standard method for the identification of the animal's true disease status.
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Electron microscopy (EM), a gold-standard method for determination of autophagy, revealed that autophagic vacuoles (autophagosomes/autolysosomes) were dramatically increased in COPD lung tissues, whereas little vacuole formation was evident in control tissues (Fig. 1B).
qRT-PCR is often considered a "gold-standard" method of measuring gene expression.
Although limited by the absence of a gold-standard method to confirm LTBI, in our study QFT has shown a higher response than TST, which approaches statistical significance.
The euglycemic-hyperinsulinemic clamp (EHC) is a gold-standard method for measuring insulin sensitivity (IS) but the complexity of the technique limits its use in research.
Currently, there is no consensus on a gold-standard method to isolate/purify EVs according to the type of biofluid or EV type desired [ 30].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com