Miron et al have demonstrated that the popular strategy of selecting genes with the greatest fold-increase generally fails as a global validation strategy while a random selection of genes for validation (10 25 genes) is a more robust technique [ 25].
Given the lack of a consensus gold standard for methylation quantification, we consider that the global validation of the Methylation array Platform results with an additional technique (57 90%) was quite appropriate but far from perfect, indicating that a validation would be especially relevant for each potentially pathogenic aberration.
The current transaction can be added to the blockchain only upon global validation of its history.
This global validation of the model provides important evidence that the frequencies of utilization of the reactions during the simulations are qualitatively consistent with the metabolic fluxes observed experimentally.
For all information pertinent to RT-qPCR validation of microarray results see Additional file 4. Microarray and qPCR fold changes (FCs) among all five tissues for ten target genes were compared in Log2 space using the concordance correlation coefficient (CCC), proposed for global validation of microarray experiments [ 54].
This reconciliation method is described and a global methodology including validation of measurement, elimination of irrelevant points and validation of the reconciliation method is proposed.
So, when the "likes" and affirming responses to our carefully selected selfies come in large numbers in a given week, the satisfaction of that kind of global validation is immeasurable.
Some examples of global validation based on reactor data are depicted, especially the calculation of a natural convection test in Phenix reactor.
In this sense, while adding RT-qPCR data on a few genes may still be sufficient for publication under current standards, our microarray experiments would support that global validation to confirm larger sets of genes may be more appropriate, especially when gene lists derived from these studies are exploited for data integration and systems modeling.
Döll, P., Kaspar, F. & Lehner, B. A global hydrological model for deriving water availability indicators: model tuning and validation.
