Exact(2)
Both fluorescent microscopy and scanning electron microscopy indicated that the cell morphology differed on the various materials (HA fibers on Matrigel, pure Matrigel and a glass control).
The cellular interaction and response of the Vero cell line with an in-house synthesised AAO membrane, a commercially available membrane, and a glass control were assessed by investigating cell adhesion, morphology, and proliferation over a 72 h period.
Similar(58)
Surface area fouling was reduced by 90% relative to a cleaned glass control substrate.
Position 15 was left empty (nothing at all was applied) and served as a negative glass control.
OECs attached onto the tissue culture glass control displayed a healthy, flat appearance with many filopodial extensions.
The number of viable Vero cells proliferating over the surface of each respective membrane and glass control was quantified over a 72 h period using a standard cell counting technique.
The cellular response of the cell line to both porous membranes types and the glass control surface was evaluated over a 72 h period using a cell proliferation assay.
Furthermore, all GFAP/α-tubulin intensity ratios were divided by the average GFAP/α-tubulin intensity ratio of the glass control group in order to a) normalize the average value of the control group to a value of 1 and b) provide experimental GFAP/α-tubulin tubulin intensity ratios as a fold-increase relative to the glass control.
Also at this point a levelling trend in both the in-house AAO membrane and glass control could also be seen.
The gene expression is relative to GAPDH, and normalized to the conventional PLL-coated glass control [70].
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