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In this context we also point out that a PBN formulation can be used to exactly obtain the translation rate for a given initiation rate (without simulation), and so can be used more effectively in the iterative process, than simulation.
A body of experimental evidence has been used to support the view that in bacteria the two sister replisomes derived from a given initiation event are associated into a replication factory (Adachi et al., 2005; den Blaauwen et al., 2006; Lemon and Grossman, 1998, 2000; Molina and Skarstad, 2004).
We also point out that the steady state translation rate and protein concentration can be solved from the first method by simulating the translation process for a given initiation rate, determining the translation rate, then determining the protein concentration and iterating.
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1 2 School-based interventions have been recommended to be implemented to reduce this burden given initiation of such drug use typically occurs during adolescence, 3 and schools provide almost universal access to adolescents for prolonged periods.
The loss of cross-sectional area of steel is computed for a given corrosion initiation time in the next section.
We thus link the two processes and show that the import rate through a pore not only depends on the location of the initiation tag but also on the mechanical properties of the protein when averaged over all the possible geometries that are relevant for a given translocation initiation site.
Our results are contrary to both central assumptions of the factory model: that the two sister forks derived from a given replication initiation remain close together and that they occupy a fixed cell position into which parental DNA enters and newly replicated DNA exits.
The other seven mutations gave initiation biases that were not significantly different from that of the original two-ARS plasmid.
Persistence was defined as the total time that any patient was prescribed a given drug, from initiation of treatment to the end of the last supplied prescription, without intervening discontinuation of that drug.
The above mechanisms not only help keep the cytoplasmic Ca2+ level at bay so that any increase from basal levels can be read as a signal of metabolic or environmental change, but also limit the free diffusion of Ca2+ so that the Ca2+ signals remain local, unless a given threshold for initiation of a propagating, global Ca2+ wave is breached (30).
Each cross in the figure represents the COP value at a given percentage of gait initiation and walking (shaded areas represent double support phase).
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