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To achieve this, we separated the two reactions physically by melting a gem of AmpliWax® in-between the two layers, so that the PCR master mix was locked in the bottom of the tube and separated from the Phi29 amplification reaction that was placed on top of the AmpliWax®-seal.
Briefly, in Amp-PCR, a random unbiased isothermal Phi29 amplification (Repli-g Midi, Qiagen) reaction was combined with a virus-specific real-time PCR and the two reactions physically separated by a gem of AmpliWax® melted in-between the two layers (Figure 1A).
It's a gem, pitched somewhere between the English baroque found on high-priced rarities like Mandy More's But That Is Me and the singer-songwriter slot on That's Life.
For years he had suffered the inefficiencies of the age-old business, where seven layers of middlemen can jack up the price of a gem 1,000% between original wholesale and final retail prices.
For years he suffered the inefficiencies of the age-old business, where seven layers of middlemen can jack up the price of a gem 1,000% between wholesale and final retail prices.
But once in a while, a culinary gem appears between the Starbucks and the Burger King, elevating terminal eating with the fresh, the organic, the artful plate garnished with sweet tomato chutney and a sprig of mint.
He's a gem.
Allotments is a gem.
It is a gem.
He was a gem.
Carmona, is a gem.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com