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Allelic amino acid sequences were aligned and each site containing a gap was removed from the alignment (indel sites are illustrated in Additional file 1).
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Ambiguous regions and all sites including a gap were removed from the alignment.
Columns in which the seed had a gap were removed.
Alignment positions for which a fraction > 0.95 of the individuals have a gap are removed from the alignment.
Afterwards, all positions containing a gap were removed from the alignment and the amino acids were replaced by their corresponding codons.
Shortly, the reported numbers represent the ratio of identities to the length of the longer of the two sequences after positions where both sequences contain a gap are removed.
The protein alignments were back-translated into nt sequences using a perl script and all gaps were removed.
Additionally, the sequences with the region of gap were removed.
In contrast, when the interaction of GDIs with GAPs was removed, Rho activation level decreased and was not sustained.
Positions of doubtful homology or introducing phylogenetic noise due to an excessive number of gaps were removed using Gblocks [ 56] with default settings for the EI alignment.
Nucleotide positions with a proportion of ≥60% gaps were removed for the sake of clarity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com