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This was based on the transcriptional activity of a fusion constructed on the assumption of a luxS transcription start that was not experimentally supported [21].
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We also describe a fusion construct of the thymidine-activating dCK to the cytoplasmic tail-truncated LNGFR molecule and applications to in vivo eradication of primary human T cells.
In this NMR study, by successful expression and purification of the E2 transmembrane domain as a fusion construct we have determined the global fold and characterized backbone motions for this peptide incorporated in phospholipid micelles.
Two proteins in a fusion construct were separated by an additional -Ser-Ser linker peptide.
We developed a vaccine comprising a fusion construct designated HAV, containing components of two secreted and two cell surface Mycobacterium avium subspecies paratuberculosis proteins.
When CaM was expressed as a fusion construct with GFP, the protein also showed cytosolic staining in addition to CV localization (Fig. 6A).
Here we report the development of a vaccine against MAP comprising two secreted and two cell surface mycobacterial components incorporated in a fusion construct designated HAV and delivered in recombinant DNA, human Ad5 and MVA vectors.
For production of variants with linkers of 3 or 16 amino acids, a fusion construct consisting of the N-terminal domain of PABPN1 and CspB served as a template [19].
A fusion construct containing murine VE-cadherin cDNA in frame with mCherry [23] at the C-terminus was subcloned into pTolfli1epDest vector under the endothelial fli1 enhancer/promoter flanked by Tol2 transposable elements [24].
This discrepancy is likely due to the increased exposure of Gcg-XTEN in vivo, suggesting that reduced specific activity is not likely to strongly affect overall efficacy of a fusion construct.
ORCTL3-ECFP was generated as a fusion construct with a pcDNA3 vector backbone.
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