Furthermore, recently developed high-throughput protein biomarker screening methods, such as multiple reaction monitoring, could be applied to a broader cohort of samples for a further validation of the observed biological changes (Sandhu et al. 2008; Whiteaker et al. 2007).
For this, a further validation of radius quantification was carried out against a partially rescanned portion of the network at 8 μm voxel size, which estimated less than 10% radius error in vessels larger than 2 voxels in radius.
A further validation used for the description is the prediction of caloric parameters and the comparison thereof with measured data.
A 3-D finite element model is built for a further validation.
For a further validation, we exposed murine fibroblastic L-cells transfected with TOPflash to oscillatory fluid flow.
Indeed, to focus on genes for which differential methylation and expression could be related, we applied larger sample cohorts (78 paired HCC samples) for a further validation using Illumina MiSeq-BSP (Fig. 2).
Among the differentially expressed miRNAs, we chose hsa-miR-363* for a further validation study because its upregulation in centenarians was detectable by qRT-PCR analysis with > 2 fold change.
However, due to the lack of suitable measurements there is still a need for further validation for offshore sites.
In summary, this work applies a systematic, yet simple, procedure to provide further validation for a small-sized model of P. pastoris, using only data from extracellular measurements.
Recently, a Caenorhabditis elegans model of ATD was reported to show reduced longevity, delayed development and decreased brood size, providing further validation for a proteotoxic effect of ATZ accumulation in a living organism (Gosai et al., 2010).
