After being left to cool, the extract was filtered using a mesh and further concentrated.
A single cross-junction ring antenna is also similar to the MINE structure, and it can support a high-field intensity at a cross junction because a field can be further concentrated at the center of the structure via attaching a ring to a cross rod dimer [42].
It's not without its carnage-based highlights, but the fact that an already intense form of combat is further concentrated into a smaller area can get to be a bit much.
In April, he won a narrow referendum that further concentrated his power and threatened the country's status as a democracy.
The virus-containing fractions were dialysed against HN buffer in Slide-A-Lyzer 3.5k dialysis cassettes (Pierce, Rockford, IL) and further concentrated on a step gradient composed of 16 μl 50% (w/w) and 32 μl 20% (w/w) sucrose in HN buffer, run for 1 h at RCFmax=8.7 × 10 g (25 k.r.p.m).r.p.m
The ethyl acetate extracts were further concentrated using a rotary evaporator.
The eluent was further concentrated in a rotary vacuum evaporator (Buchi, Switzerland) before being transferred to a glass vial.
Briefly, cells were pelleted by centrifugation at 1600×g for 3 min, suspended in 1 ml of 10 mM Tris-HCl, pH 7.5 and further concentrated into a dense pellet by centrifugation at 1600×g for 3 minutes.
The gp43 protein was eluted with 0.1 M citric acid buffer (pH 2.8), neutralized with 1 M Tris (pH 9.0), and further concentrated in a 10K Amicon apparatus (Amicon Division, Beverly, MA, USA).
The fractions were further concentrated with a rotary evaporator at 40°C under reduced pressure.
For weak or small fragments, the DNA was further concentrated using a QIAquick Gel Extraction Kit (Qiagen).
