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T6SS is typically encoded by clusters of 12 to 20 genes, with a minimum of 13 genes for production of a functional apparatus [ 42].
As persistence of any essential plastid encoded-genes requires a functional apparatus for translation, translation apparatus genes would in turn be under strong purifying selection to be retained.
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This reflects another fundamental difference in the databases' goals: while TCDB aims to transfer the full functional apparatus of a protein to all other family members, Pfam establishes relationships between individual functional modules that may be found in proteins with different functions.
Chlamydomonas can grow as a haploid and in the absence of a functional photosynthetic apparatus, allowing rapid isolation of mutants of interest.
This defect could not be rescued by centrifugation, demonstrating that a functional T1 apparatus is required for caspase-1 activation.
The mutation was introduced into the wild type background (WTM−F−; SL1344, fliGHI::Tn10) and in a mutant lacking a functional T1 apparatus (T1−M−F−; SL1344, ΔinvG fliGHI::Tn10), respectively.
In tubular epithelia of γCOP mutant embryos, the structure of both the ER and Golgi is compromised indicating that efficient luminal secretion is highly dependant on a functional secretory apparatus.
Similar observations were made for CCT knockout heterokaryon progeny that were isolated into MEPP medium that supports growth of cells lacking either a functional oral apparatus [25] or cilia [26], [27].
This finding as reported by Fields and Straley [34] was evidenced by infections with strains lacking a functional T3SS apparatus or the pLcr plasmid, which encodes the T3SS components.
The presence of a protein-coding genome, although small, necessitates the preservation of a functional translation apparatus in mitochondria.
Although our analyses did not identify any proteins likely to be involved in splicing in S. salmonicida, further sequencing and more detailed analyses are needed to determine whether introns and a functional spliceosomal apparatus are present in S. salmonicida.
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