Sentence examples for a frozen cell from inspiring English sources
The plot of the drama follows the events of the Haitian Revolution and the cruel destiny of its leader, Toussaint L'Ouverture, who dies betrayed in a frozen cell in the Jura mountains.
Although B. subtilis BA has been proven as an effective cofactor regenerator for a coupling whole-cell system in this study, for practical applications, it should be investigated further on its ability to be recycled or stored as a frozen cell pellet.
To test the applicability of these mAbs to immunoprecipitate (IP) EBNA1 from E. coli whole cell extract, a frozen cell pellet (7.5 mg wet weight) from 5 mls of induced culture was resuspended in 100 µl TE+0.1 M NaCl.
Frozen tissue samples weighing approximately 1.0 g were ground and pulverized into a fine powder in liquid nitrogen using a frozen cell crusher 'Cryo-Press™' (Microtec, Chiba, Japan), and homogenized in 10 ml of a chloroform/methanol (2∶1) mixture.
A frozen cell was thawed on ice then lysed as above.
At the fourth passage after thawing a frozen cell sample (see materials and methods), we identified two groups of cells from their karyotype (Fig. 1a).
In a subset of patients with an available frozen cell sample stored in viable conditions, surface phenotypes were evaluated on thawed PBMCs by flow cytometry (FACS Sort Becton-Dickinson, San Josè, California, USA) using directly labeled antibodies (fluorescein isothiocyanate [FITC], phycoerytrin [PE], and Peridinin-chlorophyll-protein complex cyanin 5.5 [PerCP Cy5.5]).
Genomic DNA was purified from frozen cell pellets following a published protocol67.
Total DNA was extracted from frozen cell pellets using a FastDNA® SPIN Kit for soil (MP Biomedicals LLC, Illkirch, France).
Yeast extracts were prepared by grinding the frozen cell pellets with a mortar and pestle that had been cooled with liquid nitrogen.
Total RNA was extracted from fresh or frozen cell pellets using a RiboPure Yeast Kit (Ambion).
