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After 15 min, a fresh cross section (1 mm thick, 5 mm diameter) was removed from the stem and incubated in the dark with 5 µl of a 50 µM dihydrorhodamine 123 solution (DHR123, AnaSpec Inc., Fremont, CA) for 30 min to allow ROS in the tissue to oxidize non-fluorescent DHR 123 to the fluorescent rhodamine 123.
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The tracking poll will subsequently add a fresh cross-section of 250 new interviews each week that are combined with the most recent n=250 from the previous week, as is standard practice in tracking surveys.
As a comparative control, this same analysis was performed using a fresh cross-section of a Zea mays leaf, a Kranz type C4 species that is known to have low PSII content in BS chloroplasts.
Open image in new window Fig. 1 Photographic images of a typical cross section of a fresh (a) and reduced (b) 1NiPd/Al2O3 catalysts.
Fresh stems were cut into pieces about 5 cm long, and a freehand cross section of each stem was made with a razor blade.
"It has to be a real cross section".
While we were there, it was a demographic cross section.
"I wanted a real cross section of men who dance.
The square looks something like a geological cross section.
a Hillslope cross section.
(a) Schematic cross section view.
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CEO of Professional Science Editing for Scientists @ prosciediting.com