Several grading systems have been proposed for microtia, and the most frequently used system is the Marx classification (Marx, 1926).
The Roche 454 FLX pyrosequencer was the first commercially available and is still the most frequently used system.
It is important to note that the ICD is used in clinical practice in Europe, whereas the DSM is the most frequently used system for research purposes [ 26].
Among the numerous EpCAM-based CTC detection technologies, the semi-automated CellSearch ® system is the most frequently used system and at present the only one cleared by the U.S. FDA.
Table 2 shows a summary of 5 frequently used systems.
Some frequently used systems are the "four-layer bandage" system (strictly speaking a four-component system, 4LB) and the multi-component compression that includes a "short-stretch bandage" (SSB) [ 12].
The two most frequently used systems of psychiatric classification are the International Statistical Classification of Diseases and Related Health Problems (ICD), produced by the World Health Organization, and the Diagnostic and Statistical Manual of Mental Disorders (DSM), produced by the American Psychiatric Association.
However, as these expression systems are often inadequate for expression of eukaryotic proteins, the use of alternative and less frequently used systems has been recommended [24].
The most frequently used systems in South Africa are the BacT/Alert® 3D System (bioMérieux, Durham, N.C).
Among them, the most frequently used systems include Revised European-American classification in 1994 (REAL), French-American-British system (FAB), and World Health Organization (WHO) classification in 2001 (updated in 2008) [ 105].
To allow easy reference to LEA proteins described in earlier publications, Table 2 compares the Pfam nomenclature with the two most frequently used systems proposed by Dure [ 5, 6] and Bray [ 4].
