Sentence examples for a forward assay from inspiring English sources

In contrast, we find no directional bias for dinucleotide microsatellite mutations in functionally MMR-deficient HCT116 cells, using a forward assay (Table 2).

The advantage of a reversion assay compared to a forward mutation assay is that specific types of mutational events can be studied.

In the first assay, we monitored NADPH consumption by FabG as it performs the biologically relevant reduction of the 3-oxo group of the acetoacetyl-coenzyme A (AcAcCoA) substrate analogue (Forward Assay, Figure 1B).

This compound was profiled in more detail in the Forward Assay giving an IC50 of 138 μM (Table 1).

Due to compound availability and compatibility with the biochemical assay, only 32 of these molecules were screened at a single concentration (1 mM) using the Forward Assay with AcAcCoA as substrate.

We tested the four C18H10 PAHs for mutagenicity in a forward mutation assay using S. typhimurium.

Our HCT116 cell results using a forward mutation assay differ from the previous conclusions of Yamada et al. (2002).

A forward pyrosequencing assay was generated for PEG10 to confirm the non-breed specific (G/A = R, forward) SNP and is illustrated in Figure 4, respectively.

In the budding yeast system, SMRT sequencing was used to analyze strand-exchange intermediates generated during mitotic recombination and to analyze genetic changes in a forward mutation assay.

As we were unable to detect direct physical interactions of SpFan1 with other MMR components (data not shown), we performed a forward mutation assay in order to determine the rate of spontaneous mutation in fan1-deleted cells.

In the Forward Assay, FabG catalyzes reduction of AcAcCoA with a KM = 1.0 ± 0.2 mM and kcat/ KM = (12 ± 3) × 10 M–1 s–1 in the presence of 0.8 mM NADPH (Supplementary Figure S4A).