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The majority of these tools utilize the naturally occurring genome sequence biases that exist between bacterial strains to identify regions that appear to have a foreign sequence composition (Karlin, 2001; Vernikos and Parkhill, 2008).
These two steps are: (i) integration of the shuttle vector containing a foreign sequence into the HSV-BAC genome and (ii) excision of the BAC sequences from the integrated HSV-BAC-shuttle DNA.
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In this study, we have adapted this method to efficiently generate ECTV deletion mutants lacking any foreign sequence in their genomes.
An abnormal band was amplified and sequencing of the PCR product revealed that FGFR1 was fused to a 5′ foreign sequence that was identified as exon 9 of SQSTM1 at chromosome 5q35, showing that SQSTM1 was juxtaposed to FGFR1 as the result of the chromosomal translocation.
A 19 bp foreign sequence tag inserted into the rRNA gene sequence at +92 (Fig. 2A) allowed us to discriminate transgene transcripts from endogenous rRNA transcripts (Fig. 2B).
Modifying a foreign transgene sequence to mimic coding sequences endogenous to the host plant can be thought of as a ploy to prevent triggering the host plant's defence mechanisms against foreign gene sequences.
As a result of phage insertion the genome gains a substantial amount of foreign sequence, much of which encodes phage structural proteins.
We demonstrate that engineering of the foreign sequence of a conjugate vaccine can significantly improve antibody production.
In a second type of construct, foreign sequence was inserted into the downstream NCR (NCR insert) of the HN gene, so that the number of encoded mRNAs remained unchanged.
The roX1 transgenes located at 60F and 58D contain a 1-kb insertion of foreign sequence at the extreme 3' end between roxbox2 and roxbox3.
The choice of this foreign sequence is crucial as a very strong antibody response towards it may compromise the anti-self immune response.
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