Your English writing platform
Discover LudwigExact(5)
With a fixed 5' nucleotide, these would still enable 4^4 (64) different forward and reverse primers to be used, which in combination enable the generation of 64*64 = 4069 identifiable PCR products (assuming that the reverse primer sequence is returned by the GS20).
Within the Hylobatidae, we detected the rare allele of an additional human SNP, −237 G/A, as a fixed nucleotide difference only in Symphalangus syndactylus.
The rare allele of the −307 G/A human SNP, which has been identified as a fixed nucleotide in some species of Old World monkeys [9], [49], is present in all OWM species studied here.
Similarly, we detected the rare allele of the −862 C/A human SNP as a fixed nucleotide in NWM of the Cebidae family, Cebus capucinus and the common squirrel monkey (Saimiri sciureus), indicating that the fixation of the adenine at position −862 correlates with the divergence of the Cebidae from common primate ancestors of the NWM approximately 16 20 million years ago [53], [54].
Still, with one exception (codon pair UUUUUU and pattern UnnnnU), all sub-patterns contained a fixed nucleotide in the last position of the P-site codon in the codon pair.
Similar(55)
By placing these fixed differences in the context of evolutionary relationships among primates, the ancestral nucleotide state of these fixed differences can be deduced and we can estimate the point at which a given fixed nucleotide difference diverged among the primate taxa (Figure 6).
Necterosoma darwini, which is the next closest relative to these four species, diverged from them by only 1.26 to 1.75%, but it was readily diagnosable through one fixed nucleotide change (as a comparison, N. souzannae, diverging by approximately 12%, could be diagnosed through 52 characters).
MrBayes unnecessarily estimated transitional changes (A ↔ G or C ↔ T) when RY-coding was employed, which simply imposed fixed nucleotide compositions on R (A + G) and Y (C + T) during the calculation.
In contrast, we find that exon sequences also differ between clades A and B. Of 24 segregating sites in exon 2, 10 are fixed nucleotide differences between clades.
Shared, unique, and fixed nucleotide differences at the R-loci between species were calculated using a perl script written by the authors.
Diagnosis: This species is very similar to Yaukthwa paiensis, but differs from it by more developed umbo and fixed nucleotide substitutions (Table 2).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com