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Carbamidomethylated cysteine residues were searched as a fixed modification.
Carbamidomethylation was set as a fixed modification for cysteine and oxidation of methionine as a variable modification.
Carbamidomethyl at cysteine residues was set as a fixed modification and phosphorylation (STY), oxidation at methionine, and acetylation at the N-terminus were defined as variable modifications.
Carbamidomethylation of cysteine was specified in Mascot and X!Tandem as a fixed modification.
The iodoacetamide derivative of cysteine was specified in Sequest and X!Tandem as a fixed modification.
Iodoacetamide derivative of cysteine was specified in Mascot and Sequest as a fixed modification.
Iodoacetamide derivative of cysteine was specified in both search engines as a fixed modification.
Iodoacetamide derivative of cysteine was specified in Mascot as a fixed modification.
Carbamidomethylcysteine was selected as a fixed modification and oxidation of methionine as a variable modification.
Oxidized methionines were searched as a variable modification and alkylated cysteines were searched as a fixed modification.
Variable modifications used were oxidized methionine, arginine-13C6, lysine-13C615N2, and carbamidomethyl-cysteine was a fixed modification.
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