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"The Human Genome Project had a fixed endpoint," says Anne Andrews, a nanotechnologist and neuroscientist also at UCLA.
This segment subset is defined by the point pairs e 0−e j, with e 0 being a fixed endpoint situated at the smallest line position (p 0=−R) and e j a variable endpoint situated at any position p within a line.
We then considered whether to perform a fixed endpoint or time-lapse assay.
Other potential drawbacks of a fixed endpoint immunofluorescence assays include the cost of antibodies as well as potential loss of weakly adherent mitotic cells and distortion of cellular structures during fixation.
Typically, an imaging screen for mitotic phenotypes would be performed as a fixed endpoint assay in microplates with immunofluorescence staining for DNA and indicators of mitotic arrest and perhaps also apoptosis [20].
Interestingly, prior reports suggest that slow administration can decrease [ 10, 11] or increase [ 12, 13] the amount of propofol used to achieve a fixed endpoint.
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To the best of our knowledge, such an idea was first used to solve deterministic differential Riccati equations in (Reid 1946) (though in that paper, the author considered the second variation for a nonsingular nonparametric fixed endpoint problem in the calculus of variations rather than an optimal control problem).
In addition, we give an NC algorithm for finding a Hamiltonian path with one fixed endpoint.
In solving the cycle problem we also solve the problem of finding a Hamiltonian path with one fixed endpoint.
The control is put on the fixed endpoint.
However, fixed endpoint imaging would limit our ability to quantify both the mitotic arrest and apoptosis responses, since they are separated by ∼24 hours.
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